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By Abelson J.N., Simon M.I., Phillips I.M.

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M. Gown, B. Winther, L. Meuse, L. K. Cohen, A. R. Thompson, and M. A. Kay, Nature Genet. 16, 270 (1997). 52 T. R. Flotte, B. Carter, C. Conrad, W. Guggino, T. Reynolds, B. Rosenstein, G. Taylor, S. Walden, and R. Wetzel, Hum. Gene Ther. 7, 1145 (1996). 42 ANTISENSERECEPTORTARGETS [3] using AAV gene delivery in patients. Ye and colleagues53 used rapamycininducible expression of erythropoietin delivered in recombinant AAVs. The intramuscular injection into mice or rhesus monkeys caused increased hematocrit and erythropoietin levels after iv rapamycin administration for up to 4 months.

Li, X. Xu, J. Gu, and R. M. Hoffman, Anticancer Res. 16(4A), 1993 (1996). 29j. A. Roth, D. Nguyen, D. D. Lawrence, B. L. Kemp, C. H. Carrasco, D. Z. Ferson, W. K. Hong, R. Komaki, J. J. Lee, J. C. Nesbitt, K. M. Pisters, J. B. Putnam, R. Schea, D. M. Shin, G. L. Walsh, M. M. Dolormente, C. I. Han, F. D. Martin, N. Yen, K. Xu, L. C. Stepehens, T. J. McDonnell, T. Mukhopadhyay, and D. Cai, Nature Med. 2(9), 985 (1996). 30 E. H. Oldfield, Z. Ram, K. W. Culver, R. M. Blaese, H. L. DeVroom, and W. F.

Pellet the plasmid DNA at top speed in a microcentrifuge for 5 min at 4°. [3] ANTISENSE D N A DELIVERY FOR PROLONGED EFFECTS 45 35. Discard the supernatant, wash the pellet with 75% ethanol, and vacuum dry. 36. Dissolve the pellet in 1 ml of TE. To treat hypertension by decreasing the activity of the renin-angiotensin system we constructed plasmids for both AT1 receptor antisense (pAT1) and angiotensinogen antisense (pAGT) in the AAV-derived expression vector. We initially used a plasmid containing A A V genome and 750 bp of cDNA inserted into the A A V in the antisense direction downstream from the A A V promoter.

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Antisense Technology: Applications Part B by Abelson J.N., Simon M.I., Phillips I.M.

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